Ukuhlaziywa komkhondo wamasampula oketshezi kunohlu olubanzi lwezinhlelo zokusebenza kusayensi yempilo nokuqapha kwemvelo.Kulo msebenzi, sithuthukise i-photometer ehlangene futhi engabizi esekelwe kuma-metal waveguide capillaries (MCCs) ukuze kunqunywe ukumuncwa kwe-ultrasensitive.Indlela yokubona ingakhuliswa kakhulu, futhi ibe yinde kakhulu kunobude bomzimba be-MWC, ngoba ukukhanya okuhlakazwe yizindonga ezisemaceleni zensimbi ezibushelelezi kungaqukethwe ngaphakathi kwe-capillary kungakhathaliseki ukuthi i-engeli yesigameko.Ukugxila okuphansi njengokungu-5.12 nM kungafinyelelwa kusetshenziswa ama-reagents avamile e-chromogenic ngenxa yokukhulisa ukukhanya okungahlangene kwemigqa nokushintsha kwesampula okusheshayo nokutholwa kweglucose.
I-Photometry isetshenziswa kakhulu ekuhlaziyeni umkhondo wamasampula oketshezi ngenxa yobuningi bama-reagents e-chromogenic atholakalayo kanye namadivayisi we-semiconductor optoelectronic1,2,3,4,5.Uma kuqhathaniswa ne-cuvette-based absorbance determination, liquid waveguide (LWC) capillaries reflect (TIR) ngokugcina ukukhanya kwe-probe ngaphakathi kwe-capillary1,2,3,4,5.Kodwa-ke, ngaphandle kokuthuthukiswa okuqhubekayo, indlela ye-optical iseduze kuphela nobude bomzimba be-LWC3.6, futhi ukwandisa ubude be-LWC ngaphezu kwe-1.0 m kuzobhekana nokunciphisa ukukhanya okunamandla kanye nengozi enkulu yamabhamuza, njll.3, 7. Mayelana kuseli elihlongozwayo le-multi-reflection ukuze kuthuthukiswe indlela ye-optical, umkhawulo wokutholwa uthuthukiswa kuphela ngesici esingu-2.5-8.9.
Njengamanje kunezinhlobo ezimbili eziyinhloko ze-LWC, okuyi-Teflon AF capillaries (enenkomba ye-refractive engu-~1.3 kuphela, ephansi kunaleyo yamanzi) nama-silica capillaries ahlanganiswe ne-Teflon AF noma amafilimu ensimbi1,3,4.Ukuze kuzuzwe i-TIR ekuxhumaneni phakathi kwezinto ze-dielectric, izinto ezinenkomba ephansi ye-refractive kanye nama-angles high incidence angles ziyadingeka3,6,10.Ngokuphathelene nama-capillary e-Teflon AF, i-Teflon AF iphefumula ngenxa yesakhiwo sayo esinezimbotshana3,11 futhi ingakwazi ukumunca izinto ezincane kumasampula amanzi.Kumacapillari equartz ambozwe ngaphandle nge-Teflon AF noma insimbi, inkomba ye-refractive ye-quartz (1.45) iphakeme kunamasampula amaningi oketshezi (isb. 1.33 wamanzi)3,6,12,13.Kuma-capillaries ahlanganiswe nefilimu yensimbi ngaphakathi, izakhiwo zokuthutha ziye zafundwa14,15,16,17,18, kodwa inqubo yokumboza iyinkimbinkimbi, ubuso befilimu yensimbi inesakhiwo esinzima nesinezimbotshana4,19.
Ukwengeza, ama-LWC okuhweba (AF Teflon Coated Capillaries kanye ne-AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) anokunye ukungalungi, okufana nalokhu: kwamaphutha..Umthamo omkhulu ofile we-TIR3, 10, (2) T-isixhumi (ukuxhuma ama-capillary, amafayibha, namashubhu okungena/okuphumayo) ungabamba amabhamuza omoya10.
Ngasikhathi sinye, ukunqunywa kwamazinga eglucose kubaluleke kakhulu ekuhlonzweni kwesifo sikashukela, i-cirrhosis yesibindi nesifo sengqondo20.nezindlela eziningi zokuhlonza ezifana ne-photometry (okuhlanganisa i-spectrophotometry 21, 22, 23, 24, 25 kanye ne-colorimetry ephepheni 26, 27, 28), i-galvanometry 29, 30, 31, i-fluorometry 32, 33, 34, 35, i-optical 6 ye-optical 6 i-surface plasmon resonance.I-37, i-Fabry-Perot cavity 38, i-electrochemistry 39 kanye ne-capillary electrophoresis 40,41 njalonjalo.Kodwa-ke, eziningi zalezi zindlela zidinga imishini ebizayo, futhi ukutholwa kweglucose ekugxilweni kwe-nanomolar okuningana kuseyinselele (isibonelo, ezilinganisweni ze-photometric21, 22, 23, 24, 25, 26, 27, 28, ukugcwala okuphansi kweglucose).umkhawulo wawungu-30 nM kuphela lapho ama-nanoparticles aluhlaza okwesibhakabhaka e-Prussian asetshenziswa njengemilinganiso ye-peroxidase).Ukuhlaziywa kwe-glucose ye-nanomolar kuvame ukudingeka ezifundweni zamangqamuzana ezisezingeni lamangqamuzana ezifana nokuvinjwa komdlavuza wendlala yesinye womuntu42 kanye nokuziphatha kokulungisa i-CO2 kwe-Prochlorococcus olwandle.
Isikhathi sokuthumela: Nov-26-2022